Coding
Part:BBa_K818500:Design
Designed by: Emeraldo Jo Group: iGEM12_Groningen (2012-09-19)
Lycopene with sboA regulator
Assembly Compatibility:
- 10COMPATIBLE WITH RFC[10]
- 12COMPATIBLE WITH RFC[12]
- 21INCOMPATIBLE WITH RFC[21]Illegal BglII site found at 207
Illegal BamHI site found at 2242 - 23COMPATIBLE WITH RFC[23]
- 25INCOMPATIBLE WITH RFC[25]Illegal NgoMIV site found at 1778
Illegal NgoMIV site found at 1908
Illegal AgeI site found at 993 - 1000COMPATIBLE WITH RFC[1000]
Design Notes
The regulator sboA is derived from B. subtilis so this coding construct will work in B. subtilis.
Design Notes Regarding Cloning Strategy
The suffix restriction site is PstI - SpeI instead of SpeI - PstI. This is important regarding cloning strategy.
Source
This part is combined from biobricks: BBa_K818100 and BBa_K274200.